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Herpes Simplex Virus-specific Polypeptides Studied by Polyacrylamide Gel Electrophoresis of Immune Precipitates

D. H. Watson

Department of Virology, The Medical School, Birmingham, B15 2TJ, England

Antisera raised in rabbits against RK13 cells infected with herpes simplex virus type 1 were capable of specifically precipitating proteins synthesized after infection of BHK-21 cells with the virus. Analysis of these immune precipitates by polyacrylamide gel electrophoresis demonstrated 15 polypeptides with mol. wt. from 25 to 100000. A number of these polypeptides were not detected in purified preparations of virus particles.

Precipitates formed with two ‘monoprecipitin’ antisera were also analysed. Antiserum to the structural antigen Band II precipitated a major polypeptide of mol. wt. 47000, which was glycosylated, and corresponded in mobility to a minor component polypeptide of the herpes virus particle. The other monoprecipitin antiserum, to the herpes-specified thymidine kinase, precipitated a polypeptide with a mol. wt. of 44000. The thymidine kinase polypeptide was not glycosylated.

^* Present address: Committee on Virology, Department of Microbiology, University of Chicago, 939 East 57th St., Chicago, Illinois 60637, U.S.A.

Present address: Department of Microbiology, School of Medicine, Leeds LS2 9NL, England.

Received 18 August 1973; accepted 18 October 1973.

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