J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 22 (1974), 411-419; DOI 10.1099/0022-1317-22-3-411
© 1974 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Neubauer, R. H.
Right arrow Articles by Young, B. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Neubauer, R. H.
Right arrow Articles by Young, B. G.
Agricola
Right arrow Articles by Neubauer, R. H.
Right arrow Articles by Young, B. G.

ST-1 Bacteriophage: some Properties of a Host Range Mutant

R. H. Neubauer* and B. G. Young

Department of Microbiology, University of Maryland College Park, Maryland 20742, U.S.A.

Phage ST-1 and a host range mutant, ST-1 h, the only members of the {varphi}X174 class of bacteriophage capable of growth on male strains of Escherichia coli, were characterized with respect to their growth requirements and physical properties. These phage were found to be separable on the basis of host range and antigenic properties but not by density or morphology. Phage ST-1 showed a strict requirement for the presence of divalent cations in order for growth to occur. This requirement was shown to be at the attachment stage in the infection process. Maximal adsorption kinetics occurred in the presence of 0.016 M-CaCl2 and both ST-1 and ST-1 h showed a greater response to divalent cations than did {varphi}X174. The interaction of these phage with isolated bacterial cell walls showed that the adsorption site involved the lipid layers but not the muramic acid portion of the cell wall. The growth of phage ST-1 on the mutant bacterial strain W-3110/1 revealed a strict requirement for the presence of calcium at a period after attachment.

* Present address: Litton Bionetics, Inc., Department of Virology and Cell Biology, Kensington, Maryland 20795, U.S.A.

Received 4 September 1973; accepted 2 November 1973.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1974 by the Society for General Microbiology.