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Department of Microbiology, John Curtin School of Medical Research, Australian National University, Canberra, Australia
Recombinant and parental poliovirus particles were indistinguishable by ratezonal and isopycnic sedimentation, and by u.v. inactivation. Sensitive selective procedures, applied to ts+ recombinants to detect genetic segregation of one parent, failed to reveal any. Poliovirus genetic recombinant particles thus appear to be conventional virus particles; their significance for recombination mechanisms is discussed. Sensitivity to the growth inhibitor 2-(3-chloro-p-tolyl)-5-ethyl-1,3,4-oxadiazole is shown to depend on a product of the structural protein gene.
* Present address: Department of Developmental Biology, Research School of Biological Sciences, Australian National University.
Visiting Fellow from Lilly Research Laboratories, Indianapolis, Indiana.
Received 7 September 1973;
accepted 9 November 1973.
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