HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Langenberg, W. G. Articles by Schroeder, H. F. Search for Related Content PubMed Articles by Langenberg, W. G. Articles by Schroeder, H. F. Agricola Articles by Langenberg, W. G. Articles by Schroeder, H. F.

The Appearance of Hordeum Mosaic Virus in Wheat Leaf Cells after Fixation in Glutaraldehyde or Chromate

United States Department of Agriculture, Agricultural Research Service and Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, Nebraska, 68503, U.S.A.

Only aggregates of hordeum mosaic virus were encountered in cytoplasm of cells fixed in a fixative containing chromate (pH 7.2) and post-fixed in osmic acid. In contrast, hordeum mosaic virus inclusions in wheat leaf cells appeared after glutaraldehyde and osmic acid fixation as aggregates in well-fixed dense cytoplasm, as partly disrupted inclusions, or as loosely occurring virus in incompletely fixed cytoplasm. The results showed that use of more than one fixative in an investigation of plant virus infected tissue is useful for extending and interpreting ultrastructural observations.

Received 13 August 1973; accepted 16 November 1973.