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The Effect of an Antireceptor Serum on Mammalian Cell Lines

I. Zajac

Department of Microbiology Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania, U.S.A.

The treatment of a suspension of living HeLa cells, JJH strain, with anti-polio receptor serum resulted in the specific inhibition of these receptors. Inhibition was maintained for at least 48 h at 37 °C provided the cells remained in suspension. Allowing treated cells to adhere to a glass surface resulted in the rapid disappearance of this inhibition. No inhibition of receptors was observed when similar experiments were performed at 37 °C with HeLa (S 3) cells, a strain routinely cultured in suspension. Inhibition was demonstrated only when these cells were treated and tested at 15 °C, and was rapidly lost when the cells were incubated at 37 °C. These results suggest that the state of the cell influences the persistence of antibody on the cell surface.

^* Present address: Department of Biology, Princeton University, Princeton, New Jersey 08540.

Present address: Smith Kline and French Laboratories, L-33, 1500 Spring Garden Street, Philadelphia, Pennsylvania 19101.

Received 12 November 1973; accepted 24 January 1974.