HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Katzman, J. Articles by Wilson, D. E. Search for Related Content PubMed PubMed Citation Articles by Katzman, J. Articles by Wilson, D. E. Agricola Articles by Katzman, J. Articles by Wilson, D. E.

Newcastle Disease Virus-induced Plasma Membrane Damage

Biology Department, Rensselaer Polytechnic Institute, Troy, New York, U.S.A.

Chick embryo fibroblasts infected by Newcastle disease virus release cellular enzymes into the overlay medium. The kinetics of release were determined for two cytoplasmic enzymes, lactate dehydrogenase (LDH) and glutamic oxaloacetic transminase, and a lysosomal enzyme, betaglucuronidase. Permeability studies showed that this release was accompanied by an alteration in the permeability of the plasma membrane of infected cells. Both [¹⁴C]-sucrose and [¹⁴C]-dextran entered infected cells at times when release of enzymes was observed, 6 h post-infection, while sucrose entered cells by 4 h post-infection, before release of enzymes could be detected. The addition of cycloheximide or 2-deoxy-D-glucose to infected cells that were already leaking LDH showed that glycoprotein synthesis is required for the virus-induced release of LDH. Stabilization of membrane permeability was accompanied by stabilization of protein synthesis after virus-induced inhibition, and by a decrease in the virus antigens in the plasma membrane. It is hypothesized that the structural alterations of the plasma membrane, indicated by the presence of new virus antigens, are responsible for the functional alterations observed.

^* Present address: Department of Microbiology, University of Illinois Medical Center, Chicago, Illinois, U.S.A.

Received 15 October 1973; accepted 28 February 1974.