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Transformation of Rat Embryo Cells by Temperature-sensitive Mutants of Herpes Simplex Virus

Medical Research Council Virology Unit, Institute of Virology, University of Glasgow, Glasgow, G11 5JR

Rat embryo cells have been transformed by temperature-sensitive mutants of herpes simplex type 2 and by wild-type virus of herpes simplex types 1 and 2. Transformation was scored using the morphological criterion of focus formation and the cell changes that lead to the final focus are described.

Herpes specified antigens can be detected in at least 50% of these transformed rat embryo cells by immunofluorescence studies on unfixed material.

Transformation experiments using hamster embryo cells and HSV-2 strain 333 are also described. These transformed cells are shown to be tumourigenic in newborn hamsters. Tumour cells in turn, as a single cell suspension, produce fresh tumours in animals up to 2 weeks old with almost 100% efficiency. Tumour cells also continue to retain the herpes specific antigens.

Received 18 December 1973; accepted 28 February 1974.

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