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Integrity of Cell-bound Poly(I).Poly(C)

Rega Institute for Medical Research, University of Leuven, Leuven, Belgium

The acid-insoluble radioactivity associated with the cells following incubation of the cells with [³H]-labelled poly(I).poly(C) [poly(I).poly(C)^* for 1 h has been analysed by sucrose gradient velocity ultracentrifuging. Intact (poly(I).poly(C)^* was recovered from primary rabbit kidney (PRK) cells, partially degraded poly(I). poly(C)^* was recovered from mouse L 929 cells and completely degraded material was recovered from HeLa and VERO cells. Priming of the cells with homologous interferon did not alter the sedimentation profile of cell-associated poly(I).poly(C)^* in PRK, L 929 or HeLa cells.

^* Poly(I).poly(C)* refers to poly(I) complexed with [³H]labelled poly(C).

Received 14 January 1974; accepted 11 March 1974.