HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bablanian, R. Articles by Russell, W. C. Search for Related Content PubMed PubMed Citation Articles by Bablanian, R. Articles by Russell, W. C. Agricola Articles by Bablanian, R. Articles by Russell, W. C.

Adenovirus Polypeptide Synthesis in the presence of Non-replicating Poliovirus

Division of Virology, National Institute for Medical Research, Mill Hill, London NW7 1AA, England

The effect of superinfection of adenovirus-infected cells with poliovirus in the presence of guanidine (P+G) has been analysed by pulse-labelling of cells with [³⁵S]-methionine followed by polyacrylamide gel electrophoresis and autoradiography. The addition of P+G while reducing total polypeptide synthesis selectively inhibits cellular polypeptide synthesis and allows the visualization, particularly early in infection, of other labelled polypeptides previously obscured. Thus, at least three polypeptides i.e. ICSP-3, core-1 and ‘p-core 2’ can be detected in the absence of virus DNA synthesis. The addition of P+G also causes a retardation of the synthesis of the virus-induced polypeptides giving a clear sequence of translation viz ICSP-3: core-1: p-core 2 ICSP-1 hexon penton base fibre. Since a similar sequence can be demonstrated by the use of the inhibitor cordycepin, it seems possible that this reflects a sequence of transcription. P+G addition also effectively inhibits the formation of virus particles.

Received 14 February 1974; accepted 4 April 1974.

This article has been cited by other articles:

				Q. Xi, R. Cuesta, and R. J. Schneider Regulation of Translation by Ribosome Shunting through Phosphotyrosine-Dependent Coupling of Adenovirus Protein 100k to Viral mRNAs J. Virol., May 1, 2005; 79(9): 5676 - 5683. [Abstract] [Full Text] [PDF]