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Continuous Mouse Brain Cell Lines Chronically Infected with Japanese Encephalitis Virus

The D. I. Ivanovsky Institute of Virology, the U.S.S.R. Academy of Medical Sciences, Moscow, U.S.S.R.

Two continuous cell lines derived from suckling mouse brain cells chronically infected with Japanese encephalitis virus were studied. One cell line underwent 122 passages, and the other 35 passages. Seven single-cell clones were obtained from the first culture. Both cultures released virus into the culture medium that differed from the initial virus in resistance to ribonuclease and urea, that had no haemagglutination activity and was not neutralized by the virus-specific serum. These properties are considered phenotypic changes in as far as intracerebral passages in mice or in competent cell line restored corresponding markers of the initial virus. The results obtained are compared with other chronically virus-infected cell cultures.

Received 21 January 1974; accepted 29 March 1974.