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A Transmissible Antigen Detected in Two Cell Lines Derived from Human Tumours

Institute of Virology, Slovak Academy of Sciences, Bratislava, Czechoslovakia

R. Widmaier

Central Institute for Cancer Research, German Academy of Sciences, Berlin-Buch, GDR

J. Bubeník and M. Indrová

Institute of Experimental Biology and Genetics, Czechoslovak Academy of Sciences, Prague

. Altaner

Institute of Experimental Oncology, Slovak Academy of Sciences, Bratislava

Vesicular stomatitis virus (VSV) can form ‘pseudotype’ particles with neutralization antigen(s) derived from mouse or chicken leukaemia viruses. These pseudotype particles contain the genome of VSV, but show neutralization, host-range and interference specificity of the corresponding oncornavirus. Pseudotypes were also detected following the growth of VSV in two established cell lines derived from human tumours: in MaTu derived from mammary carcinoma and in Tu-135 derived from a sarcoma. No virus-like particles were detected in these cells by labelling with [³H]-uridine followed by sucrose density gradient sedimentation. Media from these cultures did not contain DNA polymerase. Nevertheless, the capacity to produce VSV pseudotype was transmitted to some pseudotype-negative cells following their co-cultivation with X-irradiated, pseudotype-positive cells. Cell-free filtrates did not transmit the property. An antigen detected by immunofluorescence was also consistently transmitted. The VSV pseudotype produced in cells infected by co-cultivation possessed the same neutralization specificity as VSV pseudotype produced in the original tumour cells.

Received 21 January 1974; accepted 18 April 1974.

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