J Gen Virol Try IJSEM Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 25 (1974), 125-132; DOI 10.1099/0022-1317-25-1-125
© 1974 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Drzeniek, R.
Right arrow Articles by Bilello, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Drzeniek, R.
Right arrow Articles by Bilello, P.
Agricola
Right arrow Articles by Drzeniek, R.
Right arrow Articles by Bilello, P.

Absence of Glycoproteins in Poliovirus Particles

R. Drzeniek* and Patricia Bilello

Roche Institute of Molecular Biology, Nutley, N.J., U.S.A., and Heinrich-Pette-Institut für experimentelle Virologie und Immunologie an der Universität Hamburg, Hamburg, Federal Republic of Germany

Properly purified preparations of poliovirus particles are practically free of D-galactose and N-acetyl-D-glucosamine. These sugars, however, are found in crude virus preparations, obtained by high and low speed sedimentation and by CsCl-gradient fractionation from infected HeLa cells. They are separated from the virus particle by extraction with chloroform and subsequent isopycnic sedimentation of the virus preparation. Since D-galactose and N-acetyl-D-glucosamine are regular constituents of glycoproteins, the absence of significant amounts of these sugars demonstrates the lack of glycoproteins in poliovirus particles.

Radioactive monosaccharides are recommended for the detection of cellular impurities in carbohydrate-free viruses.

A simple and rapid method for the purification of large quantities of poliovirus by ‘precipitation’ with polyethylene glycol, resuspension in CsCl solutions, extraction with chloroform and isopycnic sedimentation is described.

* Present address: Heinrich-Pette-Institut, 2 Hamburg 20, Martinistrasse 52, W. Germany.

Received 8 April 1974; accepted 18 June 1974.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1974 by the Society for General Microbiology.