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The Infection of Tobacco Protoplasts with a Variant of Brome Mosaic Virus

J. B. Bancroft

John Innes Institute, Colney Lane, Norwich NOR 70F, U.K.

Neither tobacco mesophyll protoplasts nor tobacco leaves could be infected with wild type brome mosaic virus (BMV wt) or its RNA, but both were infected when inoculated with 50 µg/ml or more of a variant of BMV (BMV V5). This is about 100 x the amount required for efficient infection by cowpea chlorotic mottle virus (CCMV). Poly-L-ornithine stimulated the infection of protoplasts with BMV V5, but was not essential. BMV V5. RNA inoculum caused infection of protoplasts under conditions similar to those needed for their infection with CCMV-RNA.

^* Present address: Institute for Plant Virus Research, 959 Aobacho, Chiba, Japan.

Present address: University of Western Ontario, London, Canada.

Received 8 March 1974; accepted 24 May 1974.