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Structural Damage of Bacteriophage T₁ by Surface Inactivation

Laboratory of Microbiology, State University, Catharijnesingel 59, Utrecht, The Netherlands, and National Institute of Public Health, P.O. Box 1, Bilthoven, The Netherlands

The structure of bacteriophage T₁ was examined after inactivation by rotating the virus suspension in a rotating bulb or by bubbling gas through the suspension. After inactivation the [³H]-label of the nucleic acid was located in CsCl and sucrose gradients at the site of free DNA. The small fraction of phage which is resistant to surface inactivation had the same sedimentation coefficient and temperature sensitivity as the control phage, indicating that resistance is not due to agglomerate formation. No morphological differences between phage particles before and after inactivation could be demonstrated by electron microscopy.

^* Present address: Du Pont de Nemours, Dordrecht, the Netherlands.

Received 6 March 1974; accepted 7 June 1974.