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Chemical Modification of the Lysine-Amino Groups of Potato Virus X

Department of Biochemistry, Rothamsted Experimental Station, Harpenden, Herts AL5 2JQ, England

Potato virus X reacted with reagents commonly used for protein amino groups, and some of its properties were changed. 2,4,6-trinitrobenzenesulphonic acid, pyridoxal-5-phosphate and methyl picolinimidate altered its absorption spectrum; the last two altered its fluorescence spectrum, and the first two altered its electrophoretic mobility. These reagents did not necessarily inactivate the virus; preparations judged to contain two modified amino groups per protein subunit retained 50 to 100% of their initial infectivity. This supports the previous conclusion that PVX-Q, an infective product of PVX and an oxidized leaf phenol, contains modified lysine -amino groups.

Received 5 June 1974; accepted 24 July 1974.