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J Gen Virol 25 (1974), 337-349; DOI 10.1099/0022-1317-25-3-337
© 1974 Society for General Microbiology
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Mechanism of Interferon Induction by NDV: a Monolayer and Single Cell Study

W. R. Fleischmann, Jun.* and E. H. Simon{dagger}

Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, U.S.A.

Newcastle disease virus (NDV) stocks contain three types of particles which were tested for interferon inducing ability: (1) plaque-forming haemagglutinating particles, (2) non-plaque-forming haemagglutinating particles containing RNA and (3) non-plaque-forming haemagglutinating particles which contain no RNA. Single NDV-infected cells were isolated in microdrops and tested for interferon production as measured by protection of 30 ± 6 additional cells from challenge with mengovirus. This technique demonstrated that: (1) essentially every cell can be induced by plaque-forming NDV to produce interferon, (2) neither of the two types of non-plaque-forming haemagglutinating particles were capable of inducing detectable levels of interferon and (3) while infection with a single active virus was sufficient to induce interferon, the efficiency of induction increased with increasing multiplicity. It was concluded that some virus synthetic processes are probably required to initiate induction.

* Predoctoral fellow of the National Institutes of Health, Present address: Department of Microbiology and Biochemistry, Idaho State University.

{dagger} Direct reprint requests to Edward H. Simon.

Received 26 February 1974; accepted 8 August 1974.




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Copyright © 1974 by the Society for General Microbiology.