HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Appleyard, G. Articles by Maber, H. B. Search for Related Content PubMed PubMed Citation Articles by Appleyard, G. Articles by Maber, H. B. Agricola Articles by Appleyard, G. Articles by Maber, H. B.

Plaque Formation by Influenza Viruses in the Presence of Trypsin

Microbiological Research Establishment, Porton, Nr Salisbury, Wiltshire, U.K.

Eight strains of influenza virus were titrated as plaque-forming units in both monolayers and suspensions of chick embryo cells. In the absence of trypsin, satisfactory plaques were formed only by the A/WSN strain of virus. When trypsin was included in the overlay medium of cell monolayers, all the influenza virus strains tested produced plaques, and the plaque infectivities of all but one strain were close to the egg infectivities. Using cells suspended in agar in the presence of trypsin, four strains gave plaque infectivities indistinguishable from egg infectivities, two strains formed plaques with rather low efficiency and two strains did not produce plaques. Plaque formation was also enhanced by pronase or subtilisin. A preliminary investigation was made of the mode of action of trypsin.

Received 19 June 1974; accepted 12 August 1974.

This article has been cited by other articles:

				J. M. Ngunjiri, M. J. Sekellick, and P. I. Marcus Clonogenic Assay of Type A Influenza Viruses Reveals Noninfectious Cell-Killing (Apoptosis-Inducing) Particles J. Virol., March 15, 2008; 82(6): 2673 - 2680. [Abstract] [Full Text] [PDF]

				C. Sweet, K. J. Jakeman, K. Bush, P. C. Wagaman, L. A. Mckown, A. J. Streeter, D. Desai-Krieger, P. Chand, and Y. S. Babu Oral Administration of Cyclopentane Neuraminidase Inhibitors Protects Ferrets against Influenza Virus Infection Antimicrob. Agents Chemother., April 1, 2002; 46(4): 996 - 1004. [Abstract] [Full Text] [PDF]

				C. Scholtissek, J. Stech, S. Krauss, and R. G. Webster Cooperation between the Hemagglutinin of Avian Viruses and the Matrix Protein of Human Influenza A Viruses J. Virol., February 15, 2002; 76(4): 1781 - 1786. [Abstract] [Full Text] [PDF]