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Isolation and Preliminary Characterization of Temperature-sensitive Mutants of Sindbis Virus Strain AR339

Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England

One hundred and four temperature-sensitive mutants of Sindbis virus strain AR339 have been isolated using five different mutagens. Forty-seven mutants showed no detectable RNA synthesis at the restrictive temperature (39 °C), whereas 57 showed levels ranging from 1% to over 100% of the wild-type level. The mutants did not show complementation. The ratio of the 42S:26S RNA species in cells infected with 30 mutants showing > 10% of the wild-type level of RNA synthesis at 39 °C was measured. Of these 30 mutants, none were found which were wholly defective in production of either RNA species, although the ratio was significantly different from the wild-type for some mutants. Two mutants showed production at 39 °C of polydisperse RNA of both double-stranded and single-stranded types.

Received 20 May 1974; accepted 14 August 1974.