HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Pennington, T. H. Search for Related Content PubMed PubMed Citation Articles by Pennington, T. H. Agricola Articles by Pennington, T. H.

Vaccinia Virus Polypeptide Synthesis: sequential Appearance and Stability of Pre- and Post-replicative Polypeptides

Department of Virology, University of Glasgow, Institute of Virology, Church Street, Glasgow, G11 5JR, U.K.

The polypeptides of BSC1 cells infected with vaccinia virus and pulse labelled with [¹⁴C]-protein hydrolysate or [³⁵S]-methionine have been examined by discontinuous polyacrylamide gel electrophoresis followed by autoradiography. About 80 virus induced polypeptides were detected, 30 appearing before and 50 after the onset of virus DNA synthesis. These were termed pre- and post-replicative polypeptides respectively. The synthesis of most pre-replicative polypeptides was turned off shortly after the peak of virus DNA synthesis; when virus DNA synthesis was inhibited this turn-off did not occur and post-replicative polypeptides were not made. The synthesis of some post-replicative polypeptides started at about the time of maximal DNA synthesis, reached a peak about 1 h later, and was then turned off. Other post-replicative polypeptides whose synthesis started at this time were made for prolonged periods. The synthesis of most post-replicative polypeptides started about 1 h after the time of maximal virus DNA synthesis and continued thereafter for prolonged periods. The stability of pre- and post-replicative polypeptides was examined in pulse-chase experiments; most pre-replicative and ‘early’ post-replicative polypeptides were stable for prolonged periods, whereas, during a chase, eleven ‘late’ post-replicative polypeptides disappeared and seven new polypeptides appeared.

Received 19 July 1974; accepted 15 August 1974.

This article has been cited by other articles:

				S. Parrish and B. Moss Characterization of a Second Vaccinia Virus mRNA-Decapping Enzyme Conserved in Poxviruses J. Virol., December 1, 2007; 81(23): 12973 - 12978. [Abstract] [Full Text] [PDF]

				P. Sanz and B. Moss Identification of a transcription factor, encoded by two vaccinia virus early genes, that regulates the intermediate stage of viral gene expression PNAS, March 16, 1999; 96(6): 2692 - 2697. [Abstract] [Full Text] [PDF]

				T. Shors, J. G. Keck, and B. Moss Down Regulation of Gene Expression by the Vaccinia Virus D10 Protein J. Virol., January 1, 1999; 73(1): 791 - 796. [Abstract] [Full Text] [PDF]