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Determination of the Molecular Weight of Bovine Enterovirus RNA by Nuclease Digestion

Biochemistry Department, Queen's University, Belfast, N. Ireland

The mol. wt. of a [³²P]-labelled bovine enterovirus RNA has been determined by digesting with pancreatic RNase and separating the resulting oligonucleotides using a two-stage fractionation method on DEAE-Sephadex in 7 M-urea at pH 7.6 and pH 3.0. We have estimated the number of nucleotides as 8612 ± 55. This corresponds to a mol. wt. of 2.93 ± 0.02 x 10⁶ which is in agreement with estimates obtained by sedimentation and gel electrophoresis techniques.

Received 8 August 1974; accepted 16 September 1974.