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J Gen Virol 26 (1975), 87-94; DOI 10.1099/0022-1317-26-1-87
© 1975 Society for General Microbiology
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Miniphage — a Class of Satellite Phage to M13

J. A. Hewitt

Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305, U.S.A.

Satellite or defective bacteriophage particles can appear in extensively recycled stocks of coliphage M13. These particles, herein known as miniphage, replicate using the wild type bacteriophage as a helper. Their physical properties (u.v. spectra, sedimentation of DNA and bacteriophage, electrophoretic mobility) are described and a method for the isolation of specific satellite bacteriophage is presented.

Received 5 November 1973; accepted 16 September 1974.




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Copyright © 1975 by the Society for General Microbiology.