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Occurrence of Polyamines in Coliphages T5, X174 and in Phage-infected Bacteria

Department of Molecular Biology, Hebrew University-Hadassah Medical School Jerusalem, Israel

The polyamine spermidine and the diamine putrescine have been detected in coliphages T5 and X174. Polyamines were identified by thin-layer chromatography and mass-spectrometry of dansyl derivatives, as well as by ion-exchange chromatography. In X174 phages, polyamines were sufficient to neutralize 0.5% of DNA phosphates. The polyamine content of T5 phages depended on growth media and purification procedures, but at least 1% of DNA phosphates were neutralized by polyamines. After infection, an increase in cellular polyamine was noticed. This increase paralleled variations in ornithine decarboxylase activity.

Received 12 July 1974; accepted 1 November 1974.