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A Single Radial Haemolysis Technique for the Measurement of Influenza Antibody

A. S. Beare

^* Virology Dept., Wellcome Research Laboratories, Langley Court, Beckenham, Kent
M.R.C. Common Cold Unit, Harvard Hospital, Salisbury, Wiltshire, U.K.

A single radial haemolysis in gel technique has been developed for the detection and measurement of antibody to influenza haemagglutinin. The method combines the sensitivity of haemagglutination-inhibition with the accuracy of single radial diffusion. It is simple, quick, reproducible, does not require purified or concentrated virus, and is unaffected by non-specific inhibitors. The method is particularly suitable for the routine screening of large numbers of serum samples, and may have application also to viruses other than the influenza group.

Received 28 August 1974; accepted 6 December 1974.