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Biophysical and Biochemical Properties of Two Viruses Isolated from Aspergillus foetidus

Biochemistry Department, Imperial College of Science and Technology, London, SW7 2AZ, U.K.

Aspergillus foetidus virus S (AfV-S) and A. foetidus virus F (AfV-F) have been shown to be serogically unrelated. Amino acid compositions of the two virus capsids are compared and their capsid polypeptides have been examined by SDS-polyacrylamide gel electrophoresis. AfV-F contained one major (3) and two minor (1 and 2) polypeptides with mol. wt. 87000, 125000 and 100000, while AfV-S contained one major (1) and one minor (2) polypeptide with mol. wt. 83000 and 78000 respectively. Evidence is presented that 2 may be derived from 1 polypeptide by proteolytic degradation in vitro. The mol. wt. of AfV-F4 and AfV-S1a particles were found from sedimentation and diffusion coefficients to be 13.1 x 10⁶ and 12.4 x 10⁶ respectively. AfV-F capsid was estimated to contain 120 molecules of polypeptide 3 and one molecule each of polypeptides 1 and 2, while AfV-S capsid was estimated to contain 120 molecules of polypeptide 1.

It has been shown that S1a and S2a particles each contain a molecule of double-stranded RNA with mol. wt. 2.24 x 10⁶ (RNA-224) and 2.76 x 10⁶ (RNA-276) respectively, whereas S1b and S2b particles each contain a molecule of RNA-224 and RNA-276 respectively, together with an additional molecule of double-stranded RNA of mol. wt. 0.1 x 10⁶. Evidence is presented that S4 particles contain two molecules of RNA-224. S3 particles gave only RNA-224 on extraction, but contain the equivalent of 1 molecules of RNA-224; the nature of these particles and other possible virus replicative intermediates is discussed. Double-stranded RNA of mol. wt. 1.24 x 10⁶ was derived from a newly described particle class, Fo.

Received 11 November 1974; accepted 7 January 1975.

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