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The Effect of Dimethyladipimidate on the Stability of Cowpea Chlorotic Mottle and Brome Mosaic Viruses

Departments of Plant Sciences and Biochemistry The University of Western Ontario, London, Canada

Cross-linking lysyl residues of brome mosaic and cowpea chlorotic mottle viruses with dimethyladipimidate at pH 8.5 in the presence of MgCl₂ resulted in particles that were stable under conditions in which the viruses normally came apart. Virus substituted in the absence of MgCl₂ was not stabilized nor would it reassociate into normal particles. Substitution of isolated coat protein subunits did not, however, interfere with the assembly of capsids.

Received 12 November 1974; accepted 15 January 1975.