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J Gen Virol 27 (1975), 263-266; DOI 10.1099/0022-1317-27-2-263
© 1975 Society for General Microbiology
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Infection of Tobacco Mesophyll Protoplasts by Alfalfa Mosaic Virus

F. Motoyoshi*, R. Hull and I. H. Flack

John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.

The best conditions found for infection of tobacco mesophyll protoplasts with alfalfa mosaic virus (AMV) were pH 5.2, 2.5 µg/ml virus and 1 µg/ml poly-L-ornithine. Newly synthesized virus could be detected 15 h after inoculation and the virus concentration reached an estimated 106 to 107 particles per infected protoplast 2 days after inoculation.

* Present address: Institute for Plant Virus Research, 959 Aoba Cho, Chiba, Japan.

Received 6 August 1974; accepted 20 January 1975.




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Copyright © 1975 by the Society for General Microbiology.