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J Gen Virol 27 (1975), 319-327; DOI 10.1099/0022-1317-27-3-319
© 1975 Society for General Microbiology
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Protein Synthesis in Sendai Virus-infected Cells

V. M. Zaides, L. M. Selimova, O. P. Zhirnov and A. G. Bukrinskaya

The D. I. Ivanovsky Institute of Virology, the U.S.S.R. Academy of Medical Sciences, Moscow, U.S.S.R.

The rate of protein synthesis in chicken embryo cells infected with Sendai virus 18 to 20 h previously was about two times greater than in mock-infected controls. At this time of infection six stable virus-induced proteins, four major structural proteins (P, NH, NP and M) and two non-structural proteins (28K and 61K), were identified by electrophoresis in SDS-polyacrylamide gel of total cell extracts. The structural glycopeptide F was not detected in the infected cell extracts. Pulse-chase experiments showed that P, NP, M and 28K proteins either did not undergo any post-translational processing or the processing occurred very rapidly. By contrast, a glycopeptide NH was apparently derived from one of two unstable precursors, 69K or 63K, which were revealed only after a short pulse. The synthesis of virus-specific proteins appeared to be regulated since its rate varied for individual classes of proteins.

In nucleocapsid-like particles isolated from infected cells two major structural proteins (P and NP) were found. A minor component with a very large mol. wt. was revealed in these particles as well as in the virus particle.

Received 6 November 1974; accepted 29 January 1975.




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Copyright © 1975 by the Society for General Microbiology.