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J Gen Virol 27 (1975), 391-394; DOI 10.1099/0022-1317-27-3-391
© 1975 Society for General Microbiology
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Modifications of Cellular RNA-polymerase II after Infection with Frog Virus 3

Gabriella Campadelli-Fiume, F. Costanzo, Laura Foa'-Tomasi and M. La Placa

Istituto di Microbiologia Università di Bologna, Via San Giacomo, 12 I-40126 Bologna, Italy

RNA-polymerase II extracted from FV3-infected and uninfected BHK cells were compared by measuring their abilities to bind [3H]-amanitin and ribonucleoside triphosphates. Binding sites for [3H]-amanitin and the dissociation constant of the complex between [3H]-amanitin and RNA-polymerase II were significantly modified following FV3 infection. The apparent Km's for ribonucleoside triphosphates remained unchanged.

Received 11 November 1974; accepted 14 February 1975.




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