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J Gen Virol 28 (1975), 9-20; DOI 10.1099/0022-1317-28-1-9
© 1975 Society for General Microbiology
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Studies on Infectious Pancreatic Necrosis Virus Interactions with RTG-2 and FHM Cells: Selection of a Variant Virus-type in FHM Cells

R. Scherrer and J. Cohen

Institut National de la Recherche Agronomique, Station de Recherches de Virologie et d'Immunologie 78850 Thiverval-Grignon, France

Evidence is presented that adaptation of IPN virus (strain VR 299) to FHM cells entails the selection of a variant virus type that differs significantly from the parental, and most representative, RTG-2 virus type in being able to adsorb efficiently to, and form plaques in, FHM cells.

The plaque titre of FHM-non-adapted virus stocks (RTG-2 viruses) was reduced by at least 99.99% in FHM cells, while FHM-adapted virus stocks (FHM viruses) produced plaques at equally high titres in both RTG-2 and FHM cells.

FHM viruses and RTG-2 viruses differed also in their behaviour in RTG-2 cells in respect to plaque size distribution and growth characteristics, but both virus-types were shown to be morphologically identical, and no significant difference in reactivity against specific antiserum could be detected.

Analysis of virus in individual RTG-2 plaque isolates or plaque progeny shows that a mutation of relatively high frequency (10-4 to 10-5) probably causes the ability to infect the FHM cells efficiently. Only these mutant virus-types were found in FHM plaque isolates.

Received 30 September 1974; accepted 19 February 1975.




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Copyright © 1975 by the Society for General Microbiology.