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High Resolution Investigations with the Scanning and Transmission Electron Microscope of Haemadsorption Binding Sites of Mumps Virus-infected HeLa Cells

Heinrich-Pette-Institut für experimentelle Virologie und Immunologie an der Universität Hamburg, 2 Hamburg 20, Martinistrasse 52, Federal Republic of Germany

Specific changes at the surface of HeLa cells infected with mumps virus were investigated in parallel with the scanning and transmission electron microscope.

The distribution of haemadsorption binding sites and virus-induced antigens at cell surfaces was simultaneously studied by labelling virus-specific antigens with peroxidase-conjugated antibodies after haemadsorption.

New information was obtained upon the three-dimensional aspect of the red blood cells, the topographical distribution of their binding sites on the infected cells, and the specific structures at the cell surface which are involved in the process of haemadsorption.

Received 23 December 1974; accepted 6 March 1975.