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J Gen Virol 28 (1975), 251-254; DOI 10.1099/0022-1317-28-2-251
© 1975 Society for General Microbiology
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Hydrophobic Chromatography of Hepatitis B Surface Antigen on 1,9-Diaminononane or 1,10-Diaminodecane Linked to Agarose

A. R. Neurath, S. Lerman, M. Chen and A. M. Prince

Virology Laboratory The New York Blood Center, New York New York 10021 U.S.A.

A series of {omega}-aminoalkyl-agaroses differing in the number of carbon atoms in the alkyl chains was prepared and used for chromatography of hepatitis B surface antigen (HBsAg). HBsAg was separated from the major part of serum proteins by adsorption to columns of 1,9-diaminononane or 1,10-diaminodecane linked to agarose beads (Sepharose 4B or 2B) followed by elution with 4 M-NaSCN.

Received 6 February 1975; accepted 16 April 1975.




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