HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Logan, J. C. Articles by Pfau, C. J. Search for Related Content PubMed PubMed Citation Articles by Logan, J. C. Articles by Pfau, C. J. Agricola Articles by Logan, J. C. Articles by Pfau, C. J.

Arenavirus Inactivation on Contact with N-substituted Isatin beta-thiosemicarbazones and Certain Cations

Department of Biology, Rensselaer Polytechnic Institute, Troy, New York 12181, U.S.A.

N-methyl and N-ethyl isatin beta-thiosemicarbazones inactivate cell-free Parana and Pichinde viruses as well as three strains of lymphocytic choriomeningitis virus. This antiviral activity is abolished in the presence of the chelating agent EDTA. The rate of virus inactivation by N-methyl isatin beta-thiosemicarbazone is greatly enhanced and controlled by the addition of cupric sulphate. Divalent cations of other first transition series metals are less effective. A difference exists in the copper requirement for fast inactivation of the prototype arenavirus (lymphocytic choriomeningitis) and the Tacaribe Complex of viruses (Parana and Pichinde). In the presence of 20 µM-N-methyl isatin beta-thiosemicarbazone, LCM and Pichinde viruses can be inactivated at about the same rate if 20 µM-CuSO₄ is added to the former and 160 µM-CuSO₄ is added to the latter. Using 20 µM-N-methyl isatin beta-thiosemicarbazone and CuSO₄ the inactivation of LCM is reduced, but not eliminated, in the presence of an equal amount of infectious Pichinde virus. Crude and highly purified Pichinde virus are inactivated at the same rate when exposed to identical concentrations of N-methyl isatin beta-thiosemicarbazone and cupric sulphate. There is little detectable change in inactivation rates when Pichinde or LCM viruses are grown in a variety of different cell lines.

Received 28 February 1975; accepted 21 April 1975.

This article has been cited by other articles:

				K. E. Kinnamon, R. R. Engle, B. T. Poon, W. Y. Ellis, J. W. McCall, and M. T. Dzimianski Anticancer Agents Suppressive for Adult Parasites of Filariasis in Mongolian Jirds Experimental Biology and Medicine, May 1, 2000; 224(1): 45 - 49. [Abstract] [Full Text]