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The Synthesis of Polyadenylated Messenger RNA in Herpes Simplex Type I Virus Infected BHK Cells

Department of Virology, Medical School, Birmingham B15 2TJ, England

The pattern of polyadenylated messenger RNA (mRNA) synthesis in BHK cell monolayers, infected under defined conditions with herpes simplex type 1 virus has been investigated by polyacrylamide gel electrophoresis of pulse-labelled RNA isolated by oligo dT-cellulose chromatography. Two classes of mRNA molecules were synthesized in infected cells; these were not detected in uninfected cells. The rate of synthesis of the larger, 18 to 30S RNA class reached a maximum soon after infection and then declined, whereas the rate of synthesis of the 7 to 11S RNA class did not reach a maximum until much later and did not decline. In the presence of cytosine arabinoside, the rate of mRNA synthesis in infected cells was reduced but the electrophoretic pattern remained the same.

^* Present address: Animal Virus Research Institute, Pirbright, Woking, Surrey, U.K.

Received 4 December 1974; accepted 24 April 1975.