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Cyclic AMP Potentiation of Interferon Antiviral Activity and Effect of Interferon on Cellular Cyclic AMP Levels

R. B. Stewart

Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada

Treatment of L cells with 3 to 10 mM 3':5'-cyclic adenosine monophosphate (cAMP) in the presence of interferon was found to potentiate the development of antiviral activity. The dose response of interferon activity at various time periods in the presence and absence of cAMP indicated that potentiation of interferon activity by cAMP occurred at an early stage in the development of antiviral activity. Among the analogues of cAMP tested for interferon-potentiating activity, only the acylated derivatives were found to be active. Combined L-epinephrine and theophylline treatment of cells elevated cellular cAMP levels and also potentiated interferon-mediated antiviral activity.

Interferon was also found to elevate cAMP levels in L cells. This activity was limited to biologically active interferon and antagonized the depression of cAMP associated with vesicular stomatitis virus (VSV) infection of L cells. These observations suggest that some aspects of interferon's biological activity is associated with an alteration in cellular levels of cAMP.

^* Present address: Environment Canada, Fisheries and Marine Service, Resource Development Branch, Box 550, Halifax, N.S.

To whom reprint request should be sent.

Received 29 January 1975; accepted 2 May 1975.

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