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Polysomal RNA in Semliki Forest Virus Infected Aedes albopictus Cells

Department of Microbiology, John Curtin School of Medical Research, and Department of Developmental Biology, Research School of Biological Sciences, Australian National University, Canberra, Australia

Polysomes from Aedes albopictus cells were identified by their rapid labelling with radioactive amino acids and their sensitivity to EDTA, RNase and puromycin. The major ribosome component in cytoplasmic extracts had a sedimentation coefficient of approx. 95S and may be a ribosome dimer.

In Semliki Forest virus infected Aedes albopictus cells, 42S and 26S RNA were the major virus RNA species detected up to 10 h post infection. Virus RNA was detected in association with pre-labelled ribosomes and banded at a buoyant density of 1.55 g/ml. 42S, 38S, 33S and 26S virus RNA species were associated with polysomes.

^* Present address (and reprint address): Department of Microbiology, Queen's University, Kingston, Ontario, Canada.

Received 27 January 1975; accepted 11 June 1975.