HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Motoyoshi, F. Articles by Oshima, N. Search for Related Content PubMed Articles by Motoyoshi, F. Articles by Oshima, N. Agricola Articles by Motoyoshi, F. Articles by Oshima, N.

Infection with Tobacco Mosaic Virus of Leaf Mesophyll Protoplasts from Susceptible and Resistant Lines of Tomato

Institute for Plant Virus Research, 959 Aoba-cho, Chiba 280, Japan

A procedure was developed to isolate tomato mesophyll protoplasts in amounts sufficient for studying virus infection and multiplication. The infection of protoplasts with a tomato strain of tobacco mosaic virus occurred most efficiently when the inoculum contained 1 µg/ml virus, 1 µg/ml poly-L-ornithine and 0.01 M-phosphate buffer, pH 6.7. The efficiency of infection was influenced by the concentration of ions as well as by the pH of inoculation buffer. The virus infected protoplasts of plants homozygous for the gene Tm-2^a, which is responsible for TMV-resistance of intact leaves, and multiplied as rapidly in them as in protoplasts from the susceptible parent line. In TMV-inoculated leaf discs prepared from the resistant plants, however, the growth of virus was very limited when compared with the large yield of virus obtained from leaf discs of the susceptible plants. The possible nature of the resistance is discussed in the light of these findings.

Received 22 April 1975; accepted 17 June 1975.