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The Fate of Protein Subunits of Parainfluenza (Sendai) Virus after Adsorption to NIL8 Hamster Embryo Cells

Imperial Cancer Research Fund, London WC2A 3PX, U.K.

Adsorption of u.v.-inactivated Sendai virus on to NIL8 hamster cells causes fusion of the cells into polykaryocytes within 2 h. ‘Infected’ cells were incubated at 37 °C for periods of 10 min to 8 h and their surface proteins iodinated with [¹²⁵I] catalysed by peroxidase. Structural components of the viral envelope, such as haemagglutinin-neuraminidase (HN) and probably also the fusion protein (F) were detected in the cell membrane for periods up to 4 h post infection.

^* Permanent address: Israel Institute for Biological Research, Ness Ziona, Israel.

Received 13 May 1975; accepted 16 July 1975.