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Association of Tobacco Rattle Virus with Mitochondria

Scottish Horticultural Research Institute, Invergowrie, Dundee, Scotland

As part of a study of the way in which tobacco rattle viruses multiply, we have examined the intracellular distribution of the CAM isolate (Harrison & Woods, 1966) by means of electron microscopy of thin sections of leaves. The micrographs show a remarkable association between virus particles and mitochondria. Nothing comparable seems to have been reported with other viruses.

Tip leaves, about 2 cm. long, were taken from plants of Nicotiana clevelandii Gray 5 days after their lower leaves were inoculated with the CAM isolate. Narrow strips of leaf blade were fixed in 5% (v/v) glutaraldehyde, post-fixed in 0.1% OSO₄, stained during dehydration for 16 hr in a saturated solution of uranyl acetate in 70% (v/v) acetone, and embedded in methyl methacrylate + styrene (6:1, v/v). Sections, mostly about 600 Å thick, were cut with a Huxley ultramicrotome, using a glass knife.

Received 12 December 1967; accepted 8 February 1968.

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