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Multiple Protein Functions in the Replication of Pox Virus DNA

Department of Virology The Medical School University of Birmingham, England

The replication of pox virus DNA is known to require the prior synthesis of new proteins. It can thus be completely inhibited by the addition early after infection of either puromycin (Joklik & Becker, 1964) or p-fluorophenylalanine (FPA) (Appleyard & Zwartouw, 1965). This dependence upon protein synthesis has been related to a requirement for new enzymes and particularly the DNA polymerase which has been demonstrated in pox virus-infected cells (Magee, 1962; Green & Piña, 1962; Jungwirth & Joklik, 1965). Recently, however, Kates & McAuslan (1967) suggested that there is also need for a second type of protein function stoichiometrically related to DNA synthesis. We here draw attention to a difference in the action of puromycin and FPA which supports this interpretation and record additional findings which suggest that a third protein function may be needed to make ‘functional’ virus DNA.

Received 25 January 1968; accepted 11 March 1968.