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The Influence of Mitomycin C, Actinomycin D and Ultraviolet Light on the Replication of the Viruses of Foot-and-Mouth Disease and Vesicular Stomatitis

The Animal Virus Research Institute, Pirbright, Woking, Surrey, England

Foot-and-mouth disease virus and vesicular stomatitis virus both contain RNA and multiply in the cytoplasm (Mussgay, 1958; Mussgay & Weibel, 1963). It would be expected that their replication would not be affected by actinomycin D, which interferes with the template function of DNA (Reich et al. 1961a; Goldberg, Rabinowitz & Reich, 1962; Hurwitz et al. 1962; Kersten & Kersten, 1962), or by mitomycin C and u.v. light, which are known to destroy the cell genome (Lathja, 1960; Reich, Shatkin & Tatum, 1961b; Kersten et al. 1964). This communication presents evidence which shows that the replication of foot-and-mouth disease virus is inhibited by all three agents, whereas vesicular stomatitis virus replication is unaffected by mitomycin C or actonomycin D.

Foot-and-mouth disease virus (type O) and vesicular stomatitis virus (type Indiana) were grown in monolayers of baby hamster kidney cells (BHK 21, clone 13; Macpherson & Stoker, 1962) and assayed by the plaque method (Mowat & Chapman, 1962).

Received 13 March 1968; accepted 22 May 1968.