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Differential Effects of Sodium Dodecyl Sulphate on Strains of Carnation Ringspot Virus

Research Station, Agriculture Canada, Vancouver, British Columbia, Canada

Four isolates of carnation ringspot virus showed a reversible aggregation which was dependent on the temperature and concentration of their preparations. At 7 mg/ml, preparations of two of these isolates aggregated at room temperature but at the same concentration the other two isolates required higher temperatures (40 °C) for aggregation. A fifth isolate formed aggregates of 12 virus particles and linked aggregates. Particles of the two isolates with less tendency to aggregate were almost completely dissociated by 0.4% sodium dodecyl sulphate (SDS) at pH 5 and by 0.1% SDS at pH 7. At pH 7, an RNA component of mol. wt. 0.5 x 10⁶ was released by lower concentrations of SDS than was an RNA component of 1.5 x 10⁶. At pH 5, the sedimentation rates of unaggregated particles of the remaining three aggregating strains were unaffected by up to 15% SDS. However, treatment with 0.0075 to 0.05% SDS at pH 7 produced protein, RNA and swollen virus particles. These swollen particles were not further affected by increasing the SDS concentration to 8%.

Received 19 May 1975; accepted 23 October 1975.