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J Gen Virol 31 (1976), 135-138; DOI 10.1099/0022-1317-31-1-135
© 1976 Society for General Microbiology
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Inhibition of Protein Synthesis in Reticulocyte Lysates by Poliovirus

J. Racevskis, S. S. Kerwar and G. Koch

Roche Institute of Molecular Biology, Nutley, New Jersey 07110, U.S.A.

Addition of 1 x 1010 p.f.u. purified poliovirus to 100 µl of a rabbit reticulocyte lysate protein synthesizing system causes a complete inhibition of initiation of protein synthesis. This inhibition is not due to the viral RNA nor to any contaminants of the preparation, but is most likely caused by the viral coat protein.

Received 16 September 1975; accepted 11 December 1975.




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