HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hay, J. Articles by Timbury, M. C. Search for Related Content PubMed PubMed Citation Articles by Hay, J. Articles by Timbury, M. C. Agricola Articles by Hay, J. Articles by Timbury, M. C.

Herpesvirus Proteins: DNA Polymerase and Pyrimidine Deoxynucleoside Kinase Activities in Temperature-sensitive Mutants of Herpes Simplex Virus Type 2

Institute of Virology, University of Glasgow, Glasgow G11 5JR, U.K.

Eleven temperature-sensitive mutants of herpes simplex virus type 2 strain HG52 were examined for ability to induce DNA polymerase activity in BHK 21/C13 cells. All mutants induced DNA polymerase at a permissive temperature, (31 °C) and all DNA-positive mutants at a non-permissive temperature (38 °C). Three DNA-negative mutants induced no DNA polymerase (ts 6, ts 9) or very little DNA polymerase (ts 11), at a non-permissive temperature, while ts 1, also DNA negative, induced a little more DNA polymerase than wild-type, often at both temperatures. The DNA polymerase induced by ts 6 at 31 °C was temperature-sensitive in vivo, but only slightly so in vitro. These results were confirmed immunologically and suggest that HSV-2 codes for at least part of a DNA polymerase activity, necessary for infection, and that full expression of this enzyme involves at least three viral genes.

Received 22 September 1975; accepted 25 November 1975.