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Cauliflower Mosaic Virus: an Improved Purification Procedure and Some Properties of the Virus Particles

Department of Plant Pathology, University of California, Davis, California 95616, U.S.A.

J. D. Harvey

Department of Physics, University of Auckland, Auckland, New Zealand

A new procedure for the purification of cauliflower mosaic virus (CaMV) which involves the use of Triton X-100 and urea results in higher and more reliable yields. CaMV particles have a s_{20, w} of 208S, a D_{20, w} of 0.75 x 10^-7 cm²/s, contain 17% nucleic acid and have a mol. wt. of 22.8 x 10⁶. Virus precipitated by Mg²⁺, low pH or ethanol at moderate salt concentrations, or by polyethylene glycol, is not easily resuspended.

^* Present address: John Innes Institute, Colney Lane, Norwich, England.

Received 5 September 1975; accepted 28 November 1975.