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Morphological Components of Herpesvirus.

II. Preservation of Virus During Negative Staining Procedures

W. C. Lawrence

G. H. Cohen

^* Department of Biological Product Development, Wyeth Laboratories, Inc. Philadelphia, Pennsylvania 19101
Department of Pathobiology, School of of Veterinary Medicine
Center for Oral Health Research and Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104 U.S.A.

The disruption of envelopes and the fragmentation of capsids of equine herpesvirus type 1 observed in negatively stained samples were attributed to viral dehydration on carbon films during preparation for electron microscopy. Prior fixation of virus with OsO₄ or glutaraldehyde and subsequent application of negative stain before drying minimized envelope disruption and virtually eliminated the occurrence of capsomere sheets and broken capsids. This simple procedure significantly improves electron microscopic evaluation of herpesvirus samples.

Received 27 October 1975; accepted 17 December 1975.