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J Gen Virol 31 (1976), 245-250; DOI 10.1099/0022-1317-31-2-245
© 1976 Society for General Microbiology

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Characterization of Two Particle Types of Calf Rotavirus

Janice C. Bridger and G. N. Woode

ARC Institute for Research on Animal Diseases, Compton, Near Newbury, Berkshire, RG16 ONN, U.K.

Two distinct types of rotavirus particle were isolated in caesium chloride density gradients. The higher density particle banded at 1.38 g/ml and measured 55 ± 0.4 nm in diameter while the less dense particle banded at 1.36 g/ml, measured 66 ± 0.4 nm in diameter and appeared to possess an extra outer capsid layer. Some forms intermediate between the two types were seen. Since the two particles had a similar but not identical morphology and polypeptide composition, they were considered to be different forms of the same virus. Infectivity was associated with the larger particle which contained one more polypeptide than the smaller particle.

Received 6 November 1975; accepted 12 January 1976.


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J. M. Gilbert and H. B. Greenberg
Cleavage of Rhesus Rotavirus VP4 after Arginine 247 Is Essential for Rotavirus-Like Particle-Induced Fusion from Without
J. Virol., June 1, 1998; 72(6): 5323 - 5327.
[Abstract] [Full Text] [PDF]




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