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J Gen Virol 31 (1976), 265-269; DOI 10.1099/0022-1317-31-2-265
© 1976 Society for General Microbiology
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Electron Microscopy of Tobacco Mosaic Virus Prepared with the Aid of Negative Staining-Carbon Film Techniques

R. W. Horne, J. M. Hobart and R. Markham

John Innes Institute, Colney Lane Norwich NR4 7UH, U.K.

The negative staining-carbon technique has been applied to suspensions of freshly prepared type strain tobacco mosaic virus particles in high concentrations. Electron microscope images show that paracrystalline arrays of the virus were formed, in which large areas of rods could be viewed along their long axes and in parallel arrays in horizontal positions. High-resolution micrographs showed the protein structure units in rods photographed in both vertical and horizontal orientations.

Received 3 November 1975; accepted 13 January 1976.




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