HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Goldstein, N. O. Articles by Burness, A. T. H. Search for Related Content PubMed PubMed Citation Articles by Goldstein, N. O. Articles by Burness, A. T. H. Agricola Articles by Goldstein, N. O. Articles by Burness, A. T. H.

Requirement of an Adenylic Acid-Rich Segment for the Infectivity of Encephalomyocarditis Virus RNA

Memorial Sloan-Kettering Cancer Center, New York, New York 10021, U.S.A.

About 80% of the RNA molecules extracted from encephalomyocarditis (EMC) virus were bound by oligo(dT)-cellulose under conditions which bind poly(A) but not poly(C) nor ribosomal RNA. This shows that most EMC virus RNA molecules contain a poly(A) tract. Both bound and unbound fractions contained RNA molecules of apparently the same size when examined by sucrose gradient sedimentation, but the bound fraction contained an adenylic acid-rich segment of about 20 nucleotides long, whereas the unbound RNA did not. The bound RNA had 200 times the specific infectivity of the unbound RNA which suggests that the poly(A) tract present in EMC virus RNA is required for infectivity.

Received 3 November 1975; accepted 14 January 1976.

This article has been cited by other articles:

				Y. M. Michel, A. M. Borman, S. Paulous, and K. M. Kean Eukaryotic Initiation Factor 4G-Poly(A) Binding Protein Interaction Is Required for Poly(A) Tail-Mediated Stimulation of Picornavirus Internal Ribosome Entry Segment-Driven Translation but Not for X-Mediated Stimulation of Hepatitis C Virus Translation Mol. Cell. Biol., July 1, 2001; 21(13): 4097 - 4109. [Abstract] [Full Text] [PDF]