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Proteins Synthesized by Cucumber Cotyledons Infected with Two Strains of Cucumber Mosaic Virus

K. R. Wood

Department of Microbiology, University of Birmingham, Birmingham, B15 2TT, U.K.

Protein synthesis in cucumber cotyledons infected with two strains of cucumber mosaic virus (CMV) differing in symptom type was investigated by the technique of radioactive double-labelling of polypeptides followed by analysis on SDS polyacrylamide gels. Radioactive labelling in the presence of actinomycin D revealed several virus-stimulated proteins associated with infection. In addition to coat protein, three polypeptides with apparent mol. wt. larger than that of coat protein, together with two smaller species were detected. No qualitative difference was observed in the patterns of protein stimulation which might account for the difference in symptoms induced by the two strains.

^* Present address: European Molecular Biology Laboratory, 69 Heidelberg, Postfach 10, 2209 W. Germany.

To whom reprint requests should be addressed.

Received 2 October 1975; accepted 27 January 1976.