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Multiplication of Vesicular Stomatitis Virus in the Leafhopper Peregrinus maidis (Ashm.), a Vector of a Plant Rhabdovirus

Instituto Venezolano de Investigaciones Científicas Centro de Microbiología y Biología Celular, Apartado 1827, Caracas Venezuela

Vesicular stomatitis virus (VSV) was found to multiply efficiently in whole Peregrinus maidis (Ashm.), the leafhopper vector of maize mosaic virus (MMV), a plant rhabdovirus. Insects were inoculated with VSV by means of a microsyringe, collected at 1-day intervals and tested individually for the presence of virus. Exponential virus multiplication occurred within the first 4 days, reaching titres of 10⁶ p.f.u. per insect in days 5 to 10 after inoculation. These observations show that a common host is available to study the multiplication of a plantand an animal rhabdovirus.

Received 1 March 1976; accepted 12 March 1976.